AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE User Manual download pdf (Page 28)

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December 16, 2004 3:54 pm, GSG_Chapter_4-02.fm

Chapter 2 Designing Primers and Probes for Quantification Assays

Automatically Designing Primers for SYBR

Green Dye Assays

Primer Express Software 3.0 Getting Started Guide 21

Notes

At this point, you can:

• Manually design primers as described in “Manually Designing Primers for

SYBR

Green Dye Assays” on page 23.

• Design using the complementary sequence. For more information, see Primer

Express Software Version 3.0 Online Help.

Viewing Results Primer Express software automatically displays the Primers/Probes tab, if it finds

primers and probes. The Primers/Probes tab displays the Candidate Primers & Probes

table that contains information about the candidate primers, probes, and amplicons (see

“Figure 6. Primers/Probes tab displaying candidate primers and probes”). The forward

primer sequences are displayed using the left-to-right 5′-to-3′ convention, and reverse

primer sequence using the right-to-left 5′-to-3′ convention.

Figure 6. Primers/Probes tab displaying candidate primers and probes

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AMX DESIGN XPRESS V 1.5 - PROGRAMMER GUIDE User Manual Page 28